antibodies-online-HVEM蛋白(AA 39-202)His标签,助力细胞信号传导分析
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;">疱疹病毒进入介质(HVEM),<span style="color: black;">亦</span><span style="color: black;">叫作</span>为肿瘤坏死因子受体超家族成员14(TNFRSF14),是TNF受体超家族的人类细胞表面受体。两种TNF超家族配体淋巴毒素α(TNF-β)和LIGHT(TNFSF14)被鉴定为HVEM的细胞配体,并<span style="color: black;">起步</span>阳性信号传导。</p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;">艾美捷antibodies-online-HVEM蛋白(AA 39-202)His标签:</p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;">货号:ABIN7274831</p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;">目的</span>:HVEM (TNFRSF14)(肿瘤坏死因子受体超家族,成员14 (TNFRSF14))</p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;">蛋白类型:重组</p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;">蛋白特性:氨基酸39-202</p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;">源自</span>:人类</p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;">源自</span>:HEK-293细胞</p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;">纯化标签/结合物:这种HVEM蛋白标记有His标签。</p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;">目的:人类HVEM/TNFRSF14蛋白</p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;">序列:亮氨酸39-缬氨酸202</p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;">特性:重组人类HVEM/TNFRSF14蛋白在C末端带有His标签,由HEK293表达。它<span style="color: black;">包括</span>亮氨酸39-缬氨酸202。</p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;">纯度:<span style="color: black;">经过</span>Tris-Bis PAGE测定,纯度大于95%;<span style="color: black;">经过</span>HPLC测定,纯度大于95%。</p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;">无菌性:0.22 μm过滤</p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;">内毒素水平:<span style="color: black;">经过</span>LAL<span style="color: black;">办法</span>测定,每微克少于1EU。</p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;">格式:冻干</p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;">重组:开启前离心管。<span style="color: black;">意见</span>重组至100μg/mL以上浓度。将冻干蛋白溶于蒸馏水。</p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;">缓冲液:从0.22μm过滤的PBS (pH 7.4)溶液中冻干。<span style="color: black;">一般</span>在冻干前添加8%海藻糖<span style="color: black;">做为</span><span style="color: black;">守护</span>剂。</p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;">储存:-20°C, -80°C</p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;">储存说明:自收到之日起,在-20至-80°C下<span style="color: black;">保留</span>12个月。重组后在-80°C下<span style="color: black;">保留</span>3-6个月。重组后在2-8°C下<span style="color: black;">保留</span>2-7天。<span style="color: black;">意见</span>将蛋白分装成较小量以<span style="color: black;">得到</span>最佳储存效果。请<span style="color: black;">尽可能</span>减少冷冻-解冻循环。</p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;">有效期:12个月</p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;">分子量:18.5 kDa。<span style="color: black;">因为</span>糖基化,基于Tris-Bis PAGE结果,蛋白迁移至32-48 kDa。</p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;">典型标准曲线:</p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><img src="//q4.itc.cn/images01/20240920/b8a36ca63c484e8d91cd4e3f963d4fce.png" style="width: 50%; margin-bottom: 20px;"></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;">图:将人HVEM固定在平板上,His标签为2pg/mL(100pL/孔)。ELISA测定EC50为0.44μg/mL的人CD160、hFc标签的剂量反应曲线。</p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;">生物活性评论:</p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;">将人HVEM,His标签以2μg/ml (100μl/孔)固定在板上。<span style="color: black;">经过</span>ELISA测定人BTLA,hFc标签的剂量反应曲线,EC50为19.9ng/ml。详见测试图像。</p>
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