导师:不会用ChatGPT复现SCI图表,不要进我的实验组
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><img src="https://mmbiz.qpic.cn/mmbiz_jpg/o9IDKMS8Z5B7r9eYmaggSvJ2eWKPX5AwfrP1DYbDCwLdUMZpMepbsdvJgkoFpRJa1gRT1O7ufBMHhhsnTqcsSA/640?wx_fmt=jpeg&from=appmsg&tp=webp&wxfrom=5&wx_lazy=1&wx_co=1" style="width: 50%; margin-bottom: 20px;"></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;"><span style="color: black;">针对</span>生物信息学初学者<span style="color: black;">来讲</span>,最大挑战是缺少经验丰富的导师。</span><span style="color: black;">但ChatGPT的<span style="color: black;">显现</span>可能会改变这种<span style="color: black;">状况</span>。</span><span style="color: black;"><span style="color: black;">此刻</span>,<span style="color: black;">朋友</span>们<span style="color: black;">能够</span>把ChatGPT当作自己的助手,<span style="color: black;">指点</span>它来完成数据分析。</span></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><img src="https://mmbiz.qpic.cn/mmbiz_png/o9IDKMS8Z5AzfdVo3dPeMGkRCicpkXPSD8cDmmRQjElS1xQ7z2tkamSiaojrNJTJJSiaBhsiaiclpPZBc1LZf6vicy7Q/640?wx_fmt=png&from=appmsg&tp=webp&wxfrom=5&wx_lazy=1&wx_co=1" style="width: 50%; margin-bottom: 20px;"></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;">今天<span style="color: black;">博主</span>分享一篇收录在National Library of Medicine的文献《<span style="color: black;">Empowering Beginners in Bioinformatics with ChatGPT》</span></span></p><img src="https://mmbiz.qpic.cn/mmbiz_png/o9IDKMS8Z5AzfdVo3dPeMGkRCicpkXPSDTJYH4xaicbrvV8mBicE70d7ho5LSLk3Q1FsDibNB4YE9bzCakZQeE94gw/640?wx_fmt=png&from=appmsg&tp=webp&wxfrom=5&wx_lazy=1&wx_co=1" style="width: 50%; margin-bottom: 20px;">
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;">文献<span style="color: black;">位置</span>:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10028953/#R1</span></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;"><span style="color: black;">帮忙</span><span style="color: black;">更加多</span>生物信息/医疗<span style="color: black;">关联</span>小伙伴让ChatGPT<span style="color: black;">作为</span><span style="color: black;">咱们</span>随叫随到的“导师”</span></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;"><strong style="color: blue;">OPTICAL 模型</strong></span></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;">在本<span style="color: black;">科研</span>中,作者提出了一个迭代模型,用于微调<span style="color: black;">指点</span> ChatGPT 生成生物信息学数据分析任务代码的指令。该模型应用于<span style="color: black;">各样</span>生物信息学主题。</span></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><img src="https://mmbiz.qpic.cn/mmbiz_png/o9IDKMS8Z5AzfdVo3dPeMGkRCicpkXPSDibaXnJ17ZXJoCPr9behfPVDOq2MN6LH12aQodUSorlf5EYRDxFbbMxw/640?wx_fmt=png&from=appmsg&tp=webp&wxfrom=5&wx_lazy=1&wx_co=1" style="width: 50%; margin-bottom: 20px;"></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;">用户先<span style="color: black;">知道</span>科学问题、分析任务、计算<span style="color: black;">办法</span>和预期成果,接着在<span style="color: black;">指点</span>下撰写<span style="color: black;">仔细</span>的数据分析任务提示。之后,<span style="color: black;">她们</span><span style="color: black;">经过</span>输入提示与聊天<span style="color: black;">设备</span>人对话,生成并执行代码。</span></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;">提问流程</span></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;"><strong style="color: blue;">1.<span style="color: black;">供给</span>初始指令</strong></span></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;"><strong style="color: blue;">2.ChatGPT生<span style="color: black;">成份</span>析代码</strong></span></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;"><strong style="color: blue;">3.执行代码</strong></span></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;"> if :代码出错,<span style="color: black;">调节</span>指令</span></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;"> else:代码无误,进行下一步</span></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;"><strong style="color: blue;">4.<span style="color: black;">检测</span>结果</strong></span></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;"> <span style="color: black;">if :</span>结果<span style="color: black;">不睬</span>想,<span style="color: black;">调节</span>指令</span></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;"> <span style="color: black;">else:</span>结果达标,进入下一<span style="color: black;">周期</span></span></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;"><strong style="color: blue;">5.复审代码,确认<span style="color: black;">最后</span>指令并记录<span style="color: black;">办法</span></strong></span></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;"><strong style="color: blue;">全案例展示</strong></span></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;">作者<span style="color: black;">经过</span>以上ChatGPT指令流程成功绘制成果:</p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><img src="https://mmbiz.qpic.cn/mmbiz_png/o9IDKMS8Z5AzfdVo3dPeMGkRCicpkXPSDnRmfOHt46s6XbrRcYZhRMAia3mIz7Cz0Lx0S8DFJsvHVLh21VoDS5hg/640?wx_fmt=png&from=appmsg&tp=webp&wxfrom=5&wx_lazy=1&wx_co=1" style="width: 50%; margin-bottom: 20px;"></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><strong style="color: blue;">实战&Prompt指令</strong></p>
<h2 style="color: black; text-align: left; margin-bottom: 10px;"><span style="color: black;">短读序列比对与视觉检验在下一代测序分析中的应用</span></h2>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;">初始Prompt指令</span></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;"><span style="color: black;">做为</span>一名精通ChIP-Seq数据分析的资深生物信息学专家,你将<span style="color: black;">经过</span>编写行数尽可能少的代码来<span style="color: black;">帮助</span>我。如有<span style="color: black;">需求</span>,终止线程。<span style="color: black;">倘若</span>理解,请回复“YES”。</span></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><strong style="color: blue;"><span style="color: black;">英文Prompt</span></strong></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;">Act as an experienced bioinformatician proficient in ChIP-Seq data analysis, you will assist me by writing code with number of lines as minimal as possible. Rest the thread if asked to. Reply “YES” if understand.</span></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;">执行Prompt指令</span></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;">当前文件夹中有两个来自ChIP-Seq文库单端测序的fastq文件:ENCFF000AVS_1m.fastq.gz 和 ENCFF000AVS_10m.fastq.gz。对<span style="color: black;">每一个</span>fastq文件,将读段比对到人类参考基因组,<span style="color: black;">保留</span>为bam文件,<span style="color: black;">而后</span>转换为bigwig文件。<span style="color: black;">运用</span>的工具<span style="color: black;">包含</span>bowtie2、samtools和deepTools。bowtie2的索引文件<span style="color: black;">位置于</span>“../data/indx/bowtie2_whole_genome/”文件夹中,前缀为“hg38”。比对时<span style="color: black;">运用</span>24个CPU。请用bash语言编写代码。</span></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><strong style="color: blue;">英文Prompt</strong></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;">I have two fastq files in current folder from single-end sequencing of a ChIP-Seq library: ENCFF000AVS_1m.fastq.gz, and ENCFF000AVS_10m.fastq.gz. For each fastq file, align reads to the human reference genome, save to bam file, and then covert it to bigwig file. Tools to use: bowtie2, samtools, and deepTools. The index for bowtie2 is in the folder “../data/indx/bowtie2_whole_genome/” with “hg38” as the prefix. Use 24 CPU for the alignment. Please draft the code in bash.</span></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;"><strong style="color: blue;"><span style="color: black;">ChatGPT生成结果</span></strong></span></p><span style="color: black;"><span style="color: black;">#!/bin/bash</span></span><span style="color: black;"><span style="color: black;"># Define variables</span></span><span style="color: black;">INDEX=<span style="color: black;">"../data/indx/bowtie2_whole_genome/hg38"</span></span><span style="color: black;">CPUS=24</span><span style="color: black;">FASTQ1=<span style="color: black;">"ENCFF000AVS_1m.fastq.gz"</span></span><span style="color: black;">FASTQ2=<span style="color: black;">"ENCFF000AVS_10m.fastq.gz"</span></span><span style="color: black;"><span style="color: black;"># Align reads to reference genome using bowtie2</span></span><span style="color: black;">bowtie2 -p <span style="color: black;">$CPUS</span> -x <span style="color: black;">$INDEX</span> -U <span style="color: black;">$FASTQ1</span> | samtools sort -@ <span style="color: black;">$CPUS</span> -o ENCFF000AVS_1m.bam</span><span style="color: black;">bowtie2 -p<span style="color: black;">$CPUS</span> -x <span style="color: black;">$INDEX</span> -U <span style="color: black;">$FASTQ2</span> | samtools sort -@ <span style="color: black;">$CPUS</span> -o ENCFF000AVS_10m.bam</span><span style="color: black;"><span style="color: black;"># Convert BAM files to bigWig format using deepTools</span></span><span style="color: black;">bamCoverage -b ENCFF000AVS_1m.bam -o ENCFF000AVS_1m.bw --binSize 10 --normalizeUsing CPM</span><span style="color: black;">bamCoverage -b ENCFF000AVS_10m.bam -o ENCFF000AVS_10m.bw --binSize 10 --normalizeUsing CPM</span>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;">迭代指令</span></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;">等等,我看到你在<span style="color: black;">运用</span> "bamcoverage" 前执行了 "samtools index"。这<span style="color: black;">是不是</span><span style="color: black;">寓意</span>着在<span style="color: black;">做为</span>输入之前需要对bam文件进行排序?</span></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><strong style="color: blue;"><span style="color: black;">英文Prompt</span></strong></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;">Wait, I saw that you have "samtools index" before "bamcoverage". Does bamcoverage as bam to be sorted before using as input?</span></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><strong style="color: blue;"><span style="color: black;">运行结果:</span></strong></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><img src="https://mmbiz.qpic.cn/mmbiz_png/o9IDKMS8Z5AzfdVo3dPeMGkRCicpkXPSDh9AMBGA8scAyc9fodc5NXDVYbJNJDOQ0d8s1bibtwaDUsE1qABoIvUQ/640?wx_fmt=png&from=appmsg&tp=webp&wxfrom=5&wx_lazy=1&wx_co=1" style="width: 50%; margin-bottom: 20px;"></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;">复审Prompt指令</span></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;">我需要逐行添加注释到下面的代码中,这个代码<span style="color: black;">能够</span>很好地满足数据分析任务的<span style="color: black;">需要</span>。请等待我的代码。</span></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;">I need to insert line-by-line comments to the below code which works well to address the needs for the data analysis task. Wait for my code.</span></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><strong style="color: blue;">ChatGPT运行结果</strong></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><img src="https://mmbiz.qpic.cn/mmbiz_png/o9IDKMS8Z5AzfdVo3dPeMGkRCicpkXPSDKpmFN8zjOoaWSVkJqqB3JASw2DZUiaUowWajcCnFLYE9iaX0YicHJMHJg/640?wx_fmt=png&from=appmsg&tp=webp&wxfrom=5&wx_lazy=1&wx_co=1" style="width: 50%; margin-bottom: 20px;"></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;"><span style="color: black;">能够</span>与 ChatGPT 互动,就像与同伴<span style="color: black;">一块</span>学习<span style="color: black;">同样</span>,而同伴<span style="color: black;">亦</span>会即时做出<span style="color: black;">回复</span>。这<span style="color: black;">针对</span>生信的伙伴<span style="color: black;">来讲</span>非常有价值。</span></p><span style="color: black;"><strong style="color: blue;"><span style="color: black;">ChatGPT国内中文站</span></strong></span>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;"><strong style="color: blue;">网站<span style="color: black;">位置</span>:<strong style="color: blue;">cloud.dftianyi.com</strong></strong></span></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><strong style="color: blue;">✅️<span style="color: black;">供给</span><strong style="color: blue;">原版ChatGPT</strong>服务</strong></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><strong style="color: blue;">支持GPT-4 | GPT-4o | GPT-4o mini最新</strong></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><strong style="color: blue;"><img src="https://mmbiz.qpic.cn/mmbiz_png/o9IDKMS8Z5ABibX91N9uJAQpEKQrPfkibHB773k7WUSiczf0epsXlOibRzKsTqxubcp46p5VKEEzLN0TaiapoC1aOlQ/640?wx_fmt=other&from=appmsg&wxfrom=5&wx_lazy=1&wx_co=1&tp=webp" style="width: 50%; margin-bottom: 20px;"></strong></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><strong style="color: blue;">✅️支持<strong style="color: blue;">语音对话、数据分析、AI绘图</strong></strong></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><strong style="color: blue;"><img src="https://mmbiz.qpic.cn/mmbiz_png/o9IDKMS8Z5Bic43RbiayVB2SiaKWmXZicDoiaF3dcI0gQAkm16bBtMia29Kic1zSjrVuAwmnhicCxULiaG8LP5d877VT7Og/640?wx_fmt=other&from=appmsg&wxfrom=5&wx_lazy=1&wx_co=1&tp=webp" style="width: 50%; margin-bottom: 20px;"></strong></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><strong style="color: blue;"><strong style="color: blue;">适合人群</strong>:国内所有用户</strong></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><strong style="color: blue;">(<span style="color: black;">研究</span>/编程人<span style="color: black;">必须</span>,真的比国内AI聪明<span style="color: black;">非常多</span>)</strong></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><strong style="color: blue;">✅️GPT-4o mini<strong style="color: blue;">免费</strong></strong></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><strong style="color: blue;">✅️GPT-4 /4oTeams账号39.9元/月 (≈官网价格2折)</strong></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><strong style="color: blue;">✅️原生ChatGPT页面,<strong style="color: blue;">100次提问/每3小时</strong></strong></p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><strong style="color: blue;">✅️支持官网的所有功能</strong></p><span style="color: black;"><strong style="color: blue;"><span style="color: black;"> 外链发布社区 http://www.fok120.com/
页:
[1]