新品来袭 | 高敏微量OLINK蛋白质组赋能临床血液样本科研
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<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;">血液<span style="color: black;">做为</span><span style="color: black;">精细</span>医学<span style="color: black;">科研</span>中<span style="color: black;">要紧</span>样本类型,其可<span style="color: black;">经过</span>微创方式<span style="color: black;">得到</span>,<span style="color: black;">同期</span><span style="color: black;">亦</span>是全身各器官蛋白质蓄水池,反映机体生理病理状态信息,在<span style="color: black;">疾患</span>临床诊断等中发挥极为<span style="color: black;">要紧</span><span style="color: black;">功效</span>,<span style="color: black;">拥有</span>巨大<span style="color: black;">科研</span>价值。</p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;">然而,血液中的蛋白分布特点为组学分析带来巨大挑战:1)血液蛋白含量差异大:动态范围跨越12个数量级,对检测<span style="color: black;">办法</span>提出挑战;2)含量分布极不均:高丰度蛋白占比超过90%,<span style="color: black;">怎样</span>检出<span style="color: black;">更加多</span>低丰度蛋白<span style="color: black;">亦</span>是挑战。来自“人类<span style="color: black;">血液</span>蛋白组计划”所<span style="color: black;">运用</span>血液蛋白分析<span style="color: black;">办法</span>为<span style="color: black;">咱们</span><span style="color: black;">供给</span>了参考,除了以往<span style="color: black;">运用</span>的质谱采集等<span style="color: black;">办法</span>外,近几年<span style="color: black;">快速</span><span style="color: black;">火热</span><span style="color: black;">全世界</span>的OLINK蛋白质组学<span style="color: black;">亦</span>被纳入其中。</p>
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<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;">中科新生命现推出Olink蛋白质组新<span style="color: black;">制品</span>,实现血液样本的高敏、微量、高通量分析!</p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;">下面从技术原理,<span style="color: black;">制品</span><span style="color: black;">特殊</span>,以及应用<span style="color: black;">方法</span>带领<span style="color: black;">大众</span>一睹为快!</p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;">1、</span>技术原理</p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;">什么是OLINK蛋白质组学?</p>
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<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;">Olink底层是免疫学反应,<span style="color: black;">经过</span>一对抗体特异性识别一个<span style="color: black;">目的</span>蛋白,并借助OLINK的专利邻位延伸技术(PEA),给每一对抗体分别加上配对的核酸链,借助DNA配对的特异性,规避了多重检测时的非特异性免疫学结合带来的串扰(如上图)。<span style="color: black;">最后</span>,<span style="color: black;">仅有</span>特异性结合到<span style="color: black;">目的</span>蛋白上的抗体对,<span style="color: black;">才可</span>在尾端互补形成双链,并<span style="color: black;">经过</span>延伸形成<span style="color: black;">能够</span>被PCR扩增的核酸双链,被下游的qPCR或NGS检出。</p>
<p style="font-size: 16px; color: black; line-height: 40px; text-align: left; margin-bottom: 15px;"><span style="color: black;">日前</span>Olink<span style="color: black;">按照</span><span style="color: black;">区别</span><span style="color: black;">疾患</span>类型、<span style="color: black;">区别</span>生物过程关注的<span style="color: black;">目的</span>蛋白优化设计抗体,推出了96~3072 panel系列<span style="color: black;">制品</span>,<span style="color: black;">包含</span>心血管、癌症、炎症</p>
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